Journal: eLife

Article Title: Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism models

doi: 10.7554/eLife.98348

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal IgG2a anti-FOXA2/HNF-3β , Santa Cruz , sc-101060 , IF (1:250).

Techniques: Knock-Out, Knock-In, Control, Mutagenesis, Transfection, Construct, Plasmid Preparation, Expressing, Imaging, Recombinant, Sequencing, Multiplex Assay, Software

Journal: Cell Biology International

Article Title: Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen gels and growth factors

doi: 10.1042/cbi20100354

Figure Lengend Snippet: Figure 2 Fluorescent images of rES cell-derived DE production (A) Double labelling of differentiated cells by brachyury (red), SOX17 (green), Hoechst (nuclei, blue) and merged views of the same field following 1, 2, 3 and 4 days of differentiation. Note that expression of SOX17 was up-regulated and that of brachyury was down-regulated with duration of culture. (B) Immunofluorescence staining positive for FoxA2 (green) and Hoechst (nuclei, blue) and merged views of the same field following 1, 2 and 4 days of differentiation. (C) Double labelling of differentiated cells by CXCR4 (red), SOX17 (green), Hoechst (nuclei, blue) and merged views of the same field at 3 days. Scale bars5100 mm.

Article Snippet: Pluripotent Table 1 Primary antibodies Primary antibody Species Manufacturer Dilution AFP Mouse mAb IgG1 Sigma 1:200 Albumin Mouse mAb IgG1 Sigma 1:200 Brachyury Rabbit pAb IgG Santa Cruz Biotechnology 1:100 CK18 Mouse mAb IgG1 Sigma 1:200 CXCR4 Mouse mAb IgG1–PE R&D Systems 1:20 FoxA2 Goat pAb IgG R&D Systems 5 mg/ml SOX17 Mouse mAb IgG3 R&D Systems 10 mg/ml 776 www.cellbiolint.org N Volume 35 (8) N pages 775–781 E The Author(s) Journal compilation E 2011 Portland Press Limited stem cell markers such as OCT4 (octamer-binding factor 4) and SSEA4 (stage-specific embryonic antigen 4) were expressed in rES cells (Figures 1B and 1C).

Techniques: Derivative Assay, Expressing, Immunofluorescence, Staining

Journal: eLife

Article Title: Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism models

doi: 10.7554/eLife.98348

Figure Lengend Snippet:

Article Snippet: The following antibodies were used: mouse IgG1 anti-SOX2 (1:200 [Santa Cruz]), rabbit anti-OCT4 (1:50 [Abcam]), mouse IgG1 anti-NANOG (1:50 [Santa Cruz]), mouse IgM anti-TRA-1–81 (1:100 [Sigma]), rabbit anti-LMX1A/B (1:1000 [Millipore]), mouse IgG2a anti-FOXA2 (1:250 [Santa Cruz]), mouse IgG1 anti-OTX2 (1:100 [Santa Cruz]), rabbit anti-TH (1:500 [Sigma]), mouse IgG1 anti-MAP2 (1:1000 [Sigma]), Alexa Fluor-488/Alexa Fluor-555/Alexa Fluor-647 conjugated secondary antibodies (1:500 [Invitrogen]).

Techniques: Knock-Out, Knock-In, Control, Mutagenesis, Transfection, Construct, Plasmid Preparation, Expressing, Imaging, Recombinant, Sequencing, Multiplex Assay, Software

Journal: Advanced Materials Interfaces

Article Title: Bioinspired Hyaluronic Acid‐Based Hydrogel Fuels Bi‐Directional Lung Organoid Maturation via PIEZO1 and ITGB1 Mediated Mechanosensation

doi: 10.1002/admi.202400194

Figure Lengend Snippet: Figure 2. Validation of iPSC stemness and DE lineage commitment. A) Schematic summary of bi-directional lung organoid differentiation and maturation process. B) Bright field images showing the morphological changes of iPSCs before induction from day −3 to day 0. Representative immunofluores- cence images presenting the preservation of stemness markers (OCT4 in magenta, SOX2 in green, nuclei in blue). Scale bar: 200 μm. C) Quantification of OCT4 and NANOG expression via flow cytometry. D) Quantitative real-time PCR assay for tracking the expression of stemness genes (OCT4 and NANOG) and DE specific genes (FOXA2, GATA6, and SOX17) (n = 4, *p < 0.05, ****p < 0.0001, One-way ANOVA with Bonferroni’s multiple compar- isons test). E) Immunostaining for stemness markers including OCT4 (cyan), NANOG (green). Cell nuclei were labeled in red. Scale bar: 100 μm. F) Immunofluorescence-based identification of DE lineage markers containing SOX17 (green) and FOXA2 (white). The nuclei (blue) and F-actin (red) were stained with Hoechst 33342 and CellMask Orange Actin Tracking Stain, respectively. Scale bar:100 μm.

Article Snippet: Primary antibody Host/Isotype Supplier Catalog no. Dilution CPM Mouse/IgG1 Thermo Fisher Scientific MA5-26863 1:200 CCSP Rabbit/IgG Sigma-Aldrich SAB2108213 1:100 FOXA2 Rabbit/IgG CST 8186S 1:400 Active ITGB1 Mouse/IgG2b Millipore MAB2079Z 1:200 NMHC IIA Rabbit/IgG CST 3403S 1:50 NKX2.1 Rabbit/IgG Thermo Fisher Scientific PA5-25940 1:200 OCT4 Mouse/IgG1 Thermo Fisher Scientific MA1-104 1:500 PIEZO1 Rabbit/IgG Novus Biologicals NBP1-78446 1:100 RRAS Rabbit/IgG CUSABIO CSB-PA020513PA01HU 1:30 SPC Rabbit/IgG Thermo Fisher Scientific PA5-71680 1:25 SPC Mouse/IgG2a, κ Novus Biologicals H00006440-M01 1:50 SOX17 Mouse/IgG1 Thermo Fisher Scientific MA5-24885 1:100 SOX2 Rabbit/IgG Millipore AB5603 1:200 Subsequently, secondary antibody staining was performed at room temperature for 1 h. F-actin in cells was stained using CellMask Orange Actin Tracking Stain (A57244; Thermo Fisher Scientific, Germany).

Techniques: Biomarker Discovery, Preserving, Expressing, Cytometry, Real-time Polymerase Chain Reaction, Immunostaining, Labeling, Staining

Journal: Advanced Materials Interfaces

Article Title: Bioinspired Hyaluronic Acid‐Based Hydrogel Fuels Bi‐Directional Lung Organoid Maturation via PIEZO1 and ITGB1 Mediated Mechanosensation

doi: 10.1002/admi.202400194

Figure Lengend Snippet: Figure 3. Characterization of foregut spheroids before and after embedding in Matrigel and HA-based hydrogel. Representative immunofluorescence of primordial progenitor stage markers A) NKX2.1 (green) and B) FOXA2 (green). C) Representative bright field images showing the corresponding morphological change of the same individual AFE spheroid from day 9 (the day of encapsulation) to day 16 (8 days after encapsulation). The nuclei (cyan) and F-actin (white) were stained with Hoechst 33 342 and CellMask Orange Actin Tracking Stain, respectively. Scale bar:100 μm. Quantification of the size parameters of spheroids D) on day 9 (n = 34) and E) on day 16 (n = 25); ***p < 0.001, ****p < 0.0001, two-tailed unpaired Student t-test.

Article Snippet: Primary antibody Host/Isotype Supplier Catalog no. Dilution CPM Mouse/IgG1 Thermo Fisher Scientific MA5-26863 1:200 CCSP Rabbit/IgG Sigma-Aldrich SAB2108213 1:100 FOXA2 Rabbit/IgG CST 8186S 1:400 Active ITGB1 Mouse/IgG2b Millipore MAB2079Z 1:200 NMHC IIA Rabbit/IgG CST 3403S 1:50 NKX2.1 Rabbit/IgG Thermo Fisher Scientific PA5-25940 1:200 OCT4 Mouse/IgG1 Thermo Fisher Scientific MA1-104 1:500 PIEZO1 Rabbit/IgG Novus Biologicals NBP1-78446 1:100 RRAS Rabbit/IgG CUSABIO CSB-PA020513PA01HU 1:30 SPC Rabbit/IgG Thermo Fisher Scientific PA5-71680 1:25 SPC Mouse/IgG2a, κ Novus Biologicals H00006440-M01 1:50 SOX17 Mouse/IgG1 Thermo Fisher Scientific MA5-24885 1:100 SOX2 Rabbit/IgG Millipore AB5603 1:200 Subsequently, secondary antibody staining was performed at room temperature for 1 h. F-actin in cells was stained using CellMask Orange Actin Tracking Stain (A57244; Thermo Fisher Scientific, Germany).

Techniques: Encapsulation, Staining, Two Tailed Test